NOMO1 Polyklonaler Antikörper

NOMO1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 17792-1-AP

Synonyme

pM5 protein, PM5, Nomo, Nodal modulator 1, BOS complex subunit NOMO1

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  WB analysis of mouse colon using 17792-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17792-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 17792-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17792-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17792-1-AP (NOMO1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 17792-1-AP

  IP result of anti-NOMO1 (IP:17792-1-AP, 4ug; Detection:17792-1-AP 1:1000) with A431 cells lysate 1520ug.

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  IHC staining of human colon using 17792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

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  IHC staining of human colon cancer using 17792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17792-1-AP (NOMO1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 17792-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 17792-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 17792-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 17792-1-AP (NOMO1 Antibody) at dilution of 1:50.

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  IF Staining of MCF-7 using 17792-1-AP

  Immunofluorescent analysis of MCF-7 cells, using NOMO1 antibody 17792-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Kolongewebe, A431-Zellen, COLO 320-Zellen, humanes Hirngewebe
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Kolongewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

17792-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NOMO1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOMO1 fusion protein Ag11895
• Vollständiger Name: NODAL modulator 1
• Berechnetes Molekulargewicht: 1222 aa, 134 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC065535
• Gene symbol: NOMO1
• Gene ID (NCBI): 23420
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Clin Sci (Lond); DLX3 promotes bone marrow mesenchymal stem cell proliferation through H19/miR-675 axis.; Authors - Na Zhao