NONO Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NONO Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

37

Rezensionen (2)

Kat-Nr. : 11058-1-AP

Synonyme

55 kDa nuclear protein, NONO, NonO protein, NRB54, P54, p54(nrb), P54NRB

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Galerie der Validierungsdaten

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  WB analysis using 11058-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 11058-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11058-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11058-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11058-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11058-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11058-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11058-1-AP (NONO antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11058-1-AP

  IP Result of anti-NONO (IP:11058-1-AP, 4ug; Detection:11058-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

• 

  IHC staining of human colon cancer using 11058-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11058-1-AP (NONO antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11058-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11058-1-AP (NONO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 11058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NONO antibody (11058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 11058-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NONO antibody (11058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 11058-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug NONO antibody (11058-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HEK-293-Zellen, humanes Pankreasgewebe, humanes Skelettmuskelgewebe, Maus-Pankreasgewebe, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 31 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• IP: See 5 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 2 publications below

Produktinformation

11058-1-AP bindet in WB, RIP, IP, IHC, IF, FC, CoIP, ChIP, ELISA NONO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NONO fusion protein Ag1512
• Vollständiger Name: non-POU domain containing, octamer-binding
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC010049
• Gene symbol: NONO
• Gene ID (NCBI): 4841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Non-pou domain-containing octamer-binding protein(NONO), other named p54nrb, belongs to DBHS family, whose protein are predominately nuclear and involves in transcriptional and posttranscriptional gene regulatory functions as well as DNA repair. This family has roles in a wide range of biological processes, such as the regulation of circadian rhythm, carcinogenesis and progression of cancer. NONO forms heterodimer with SFPQ, and has been implicated in nuclear retention of A- to I-edited RNA as paraspeckle components, pre-mRAN 3'-end formation, cAMP cycling and transcriptional activation.

Publikationen

Species	Application	Title
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
human	IF	Mol Cell; Condensates induced by transcription inhibition localize active chromatin to nucleoli.; Authors - Takaaki Yasuhara
human	WB,RIP	Adv Sci (Weinh); The Conserved LncRNA DIO3OS Restricts Hepatocellular Carcinoma Stemness by Interfering with NONO-Mediated Nuclear Export of ZEB1 mRNA; Authors - Ya-Rui Hou ; KD Validated
human	IF	Hepatology; Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma.; Authors - Zhixiang Hu
human	WB,IHC,IF	Sci Adv; Interfering MSN-NONO complex-activated CREB signaling serves as a therapeutic strategy for triple-negative breast cancer.; Authors - Yuanyuan Qin ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xingyuan (Verified Customer) (07-17-2023)

The band quality was decent.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T

NONO Antibody Western Blot validation (1:2000 dilution) in 293T (Cat no:11058-1-AP)

FH

Tatyana (Verified Customer) (05-14-2023)

ICC with antibody diluted in 5% goat serum in PBST, with 2 h incubation at RT. Good nuclear staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

NONO Antibody Immunofluorescence validation (1:1000 dilution) in HeLa (Cat no:11058-1-AP)

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