NOP16 Polyklonaler Antikörper

NOP16 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31969-1-AP

Synonyme

NOP16 Nucleolar Protein, Hypothetical Protein HSPC111, HSPC185, HSPC111, HBV pre-S2 trans-regulated protein 3

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  WB analysis using 31969-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31969-1-AP (NOP16 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 31969-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NOP16 antibody (31969-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of MCF-7 using 31969-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using NOP16 antibody (31969-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, K-562-Zellen, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31969-1-AP bindet in WB, IF/ICC, ELISA NOP16 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOP16 fusion protein Ag36714
• Vollständiger Name: NOP16 nucleolar protein homolog (yeast)
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC040106
• Gene symbol: NOP16
• Gene ID (NCBI): 51491
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOP16, also known as HSPC111, is found in the intracellular protein complex. It is a ribosomal protein that is transcriptionally regulated by the oncogene c-Myc. NOP16 may be involved in the completion of ribosome synthesis, therefore, reduced NOP16 expression in cancer cells may block many biological processes. Studies indicate that NOP16 is highly expressed in more than 20 types of cancers, including colorectal, breast, and hepatocellular carcinoma, and is an important target for clinical treatment of related diseases.