NOP2 Polyklonaler Antikörper

NOP2 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10448-1-AP

Synonyme

NOL1, NOP120, NOP2, NSUN1, Nucleolar protein 1, Nucleolar protein 2 homolog, p120

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Galerie der Validierungsdaten

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  WB analysis of A2780 using 10448-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10448-1-AP (NOP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 10448-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 10448-1-AP (NOP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10448-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10448-1-AP (NOP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10448-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10448-1-AP (NOP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10448-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10448-1-AP (NOP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 10448-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 10448-1-AP (NOP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, C6-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

10448-1-AP bindet in WB, IP, IHC, IF, ELISA NOP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOP2 fusion protein Ag0498
• Vollständiger Name: NOP2 nucleolar protein homolog (yeast)
• Berechnetes Molekulargewicht: 120 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC000656
• Gene symbol: NOP2
• Gene ID (NCBI): 4839
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOL1, (synonyms: p120, NSUN1, NOP120), is a 120 kDa proliferating-cell nucleolar antigen and is the most cancer specific of the proliferation-associated nucleolar proteins identified thus far. NOL1 is expressed in G1 and peaks during the early S phase of the cell cycle and it has not been detected in benign tumors and most normal resting tissues. Overexpression of NOL1 caused the transformation of NIH 3T3 cells and expression of an antisense NOL1 construct inhibited the growth of NIH 3T3 cells. NOL is localized in a novel nucleolar microfibrillar structure, and contains, consecutively, four major domains: a basic domain, an acidic domain, a hydrophobic and methionine-rich domain, and a domain rich in cysteine and proline residues. The gene for human NOL1 was assigned to chromosome 12p13.

Publikationen

Species	Application	Title
human	WB,IF	Nucleic Acids Res; DNA damage-induced inhibition of rRNA synthesis by DNA-PK and PARP-1.; Authors - Calkins Anne S AS
human	WB	Mol Biol Cell; RRP1B targets PP1 to mammalian cell nucleoli and is associated with Pre-60S ribosomal subunits.; Authors - Chamousset Delphine D
human	WB	Transl Cancer Res; Effect of NOP2 knockdown on colon cancer cell proliferation, migration, and invasion.; Authors - Jinling Bi
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi
mouse	IF	Cell Mol Life Sci; N4-acetylcytidine of Nop2 mRNA is required for the transition of morula-to-blastocyst; Authors - Mengyun Wang
human	WB,IF,IP	Cell Rep; ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells; Authors - M Carmen Lafita-Navarro