NOP2 Polyklonaler Antikörper

NOP2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10448-1-PBS

Synonyme

Nucleolar protein 2 homolog, Nucleolar protein 1, NSUN1, NOP120, NOL1

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  WB analysis of A2780 using 10448-1-AP (same clone as 10448-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 10448-1-AP (NOP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10448-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 10448-1-AP (same clone as 10448-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 10448-1-AP (NOP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10448-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10448-1-AP (same clone as 10448-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10448-1-AP (NOP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10448-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10448-1-AP (same clone as 10448-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10448-1-AP (NOP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10448-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10448-1-AP (same clone as 10448-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10448-1-AP (NOP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10448-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10448-1-AP (same clone as 10448-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOP2 antibody (10448-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10448-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10448-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NOP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOP2 fusion protein Ag0498
• Vollständiger Name: NOP2 nucleolar protein homolog (yeast)
• Berechnetes Molekulargewicht: 120 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: BC000656
• Gene symbol: NOP2
• Gene ID (NCBI): 4839
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOL1, (synonyms: p120, NSUN1, NOP120), is a 120 kDa proliferating-cell nucleolar antigen and is the most cancer specific of the proliferation-associated nucleolar proteins identified thus far. NOL1 is expressed in G1 and peaks during the early S phase of the cell cycle and it has not been detected in benign tumors and most normal resting tissues. Overexpression of NOL1 caused the transformation of NIH 3T3 cells and expression of an antisense NOL1 construct inhibited the growth of NIH 3T3 cells. NOL is localized in a novel nucleolar microfibrillar structure, and contains, consecutively, four major domains: a basic domain, an acidic domain, a hydrophobic and methionine-rich domain, and a domain rich in cysteine and proline residues. The gene for human NOL1 was assigned to chromosome 12p13 (PMID: 23775790).

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