NOP58 Polyklonaler Antikörper

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• KD/KO Validated

NOP58 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Rezensionen (1)

Kat-Nr. : 14409-1-AP

Synonyme

HSPC120, NOL5, NOP5, NOP5/NOP58, Nucleolar protein 5

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• 

  WB analysis using 14409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14409-1-AP (NOP58 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14409-1-AP

  WB result of NOP58 antibody (14409-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NOP58 transfected HeLa cells.

• 

  IP experiment of HeLa using 14409-1-AP

  IP result of anti-NOP58 (IP:14409-1-AP, 4ug; Detection:14409-1-AP 1:8000) with HeLa cells lysate 1400 ug.

• 

  IHC staining of mouse kidney using 14409-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14409-1-AP (NOP58 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below
• RIP: See 2 publications below

Produktinformation

14409-1-AP bindet in WB, IHC, IF, IP, CoIP, RIP, ELISA NOP58 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOP58 fusion protein Ag5740
• Vollständiger Name: NOP58 ribonucleoprotein homolog (yeast)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC032592
• Gene symbol: NOP58
• Gene ID (NCBI): 51602
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The box C/D snoRNPs,is named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including: RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation [PMID:9649444,8878486]. Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins [PMID:10606270].

Publikationen

Species	Application	Title
mouse	WB	Elife; The dyskerin ribonucleoprotein complex as an OCT4/SOX2 coactivator in embryonic stem cells.; Authors - Yick W Fong
human	WB,CoIP	J Virol; Protein-RNA Interactome Analysis Reveals Wide Association of Kaposi's Sarcoma-Associated Herpesvirus ORF57 with Host Noncoding RNAs and Polysomes.; Authors - Beatriz Alvarado-Hernandez
	RIP,CoIP	iScience; BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing.; Authors - Marlene Cervantes
human	WB	Dig Dis Sci; CYP1B1-AS1 Delays the Malignant Progression of Colorectal Cancer by Binding with NOP58; Authors - Zhao Wu
human	WB,RIP,IF	EMBO Rep; N4-acetylcytidine (ac4C) promotes mRNA localization to stress granules; Authors - Pavel Kudrin
human	WB	medRxiv; New ZNHIT3 Variants Disrupting snoRNP Assembly Cause Prenatal PEHO Syndrome with Isolated Hydrops; Authors - Md Lutfur Rahman

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marlene (Verified Customer) (02-25-2019)

Immunoprecipitation:From mouse liver lysate4ug of antibody per sampleIncubated overnight at 4C

• Applications: Immunoprecipitation,
• Cell Tissue Type: Mouse liver tissue