iNOS Polyklonaler Antikörper

iNOS Polyklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, FC (Intra), CoIP, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen(207)

Kat-Nr. : 22226-1-AP

Synonyme

NOS2, EC:1.14.13.39, Hepatocyte NOS, HEP-NOS, Inducible NO synthase

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  WB analysis using 22226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22226-1-AP (iNOS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22226-1-AP

  Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22226-1-AP (NOS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  FC experiment of RAW 264.7 using 22226-1-AP

  1X10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human iNOS (22226-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in FC (Intra): mit LPS behandelte RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 143 publications below
• IHC: See 29 publications below
• IF: See 77 publications below
• CoIP: See 1 publications below

Produktinformation

22226-1-AP bindet in WB, IHC, IF, FC (Intra), CoIP, ELISA, Cell treatment iNOS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: iNOS fusion protein Ag17567
• Vollständiger Name: nitric oxide synthase 2, inducible
• Berechnetes Molekulargewicht: 1153 aa, 131 kDa
• Beobachtetes Molekulargewicht: 110-130 kDa, 65-70 kDa
• GenBank-Zugangsnummer: BC130283
• Gene symbol: iNOS
• Gene ID (NCBI): 4843
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228).

Publikationen

Species	Application	Title
mouse	IF	Theranostics; Multifunctionally disordered TiO2 nanoneedles prevent periprosthetic infection and enhance osteointegration by killing bacteria and modulating the osteoimmune microenvironment; Authors - Yangmengfan Chen
mouse,human	WB,IHC	Mol Ther; Epsin1-mediated exosomal sorting of Dll4 modulates the tubular-macrophage crosstalk in diabetic nephropathy; Authors - Jia-Lu Liu
mouse	IF	Proc Natl Acad Sci U S A; Sleep deficiency exacerbates periodontal inflammation via trigeminal TRPV1 neurons; Authors - Junhui Li
mouse	WB,IF	J Nanobiotechnology; Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy; Authors - Junlai Wan
mouse	IHC	Cancer Lett; Inducible nitric oxide synthase (iNOS)-activated Cxcr2 signaling in myeloid cells promotes TGFβ-dependent squamous cell carcinoma lung metastasis; Authors - Xing Li
mouse	WB,IF	ACS Appl Mater Interfaces; Extracellular Matrix-Mimicking Hydrogel with Angiogenic and Immunomodulatory Properties Accelerates Healing of Diabetic Wounds by Promoting Autophagy; Authors - Yujie Pan