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NOS3 Rekombinanter Antikörper

NOS3 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242543E10

Kat-Nr. : 85020-4-PBS

Synonyme

eNOS, EC-NOS, ECNOS, EC:1.14.13.39, cNOS

Filter:
  • Western Blot (WB) analysis of bEnd.3 cells using NOS3 Recombinant antibody (85020-4-RR)

    WB analysis of bEnd.3 using 85020-4-RR (same clone as 85020-4-PBS)

    bEnd.3 cells were subjected to SDS PAGE followed by western blot with 85020-4-RR (NOS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85020-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using NOS3 Recombinant antibody (85020-4-RR)

    WB analysis of HUVEC using 85020-4-RR (same clone as 85020-4-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 85020-4-RR (NOS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85020-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85020-4-RR

    Affinity and Kinetic Characterization of 85020-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85020-4-RR against Human NOS3 were performed. The affinity constant is 3.17 nM.

Geprüfte Anwendungen

Produktinformation

85020-4-PBS bindet in WB, Indirect ELISA NOS3 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen NOS3 fusion protein Ag25712
Vollständiger Name nitric oxide synthase 3 (endothelial cell)
Beobachtetes Molekulargewicht140 kDa
Gene symbol eNOS
Gene ID (NCBI) 4846
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endothelial NOS(eNOS), also known as nitric oxide synthase 3(NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. NOS3 has 3 isoforms of 68, 69, 133 kDa. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine.