NOSTRIN Polyklonaler Antikörper

NOSTRIN Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 20116-1-AP

Synonyme

eNOS-trafficking inducer, eNOS trafficking inducer, DaIP2, BM247 homolog

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  WB analysis using 20116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20116-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 20116-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 20116-1-AP

  IP result of anti-NOSTRIN (IP:20116-1-AP, 4ug; Detection:20116-1-AP 1:500) with human placenta tissue lysate 1600ug.

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  IHC staining of human heart using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20116-1-AP (NOSTRIN Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20116-1-AP (NOSTRIN Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse testis using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 20116-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 20116-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NOSTRIN antibody (20116-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 20116-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NOSTRIN antibody (20116-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20116-1-AP

  Immunofluorescent analysis of HepG2 cells using 20116-1-AP (NOSTRIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Plazenta-Gewebe, Mausherzgewebe, Mauslebergewebe, Mauslungengewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Leberzirrhosegewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

20116-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA NOSTRIN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOSTRIN fusion protein Ag13989
• Vollständiger Name: nitric oxide synthase trafficker
• Berechnetes Molekulargewicht: 506 aa, 58 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC014189
• Gene symbol: NOSTRIN
• Gene ID (NCBI): 115677
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOSTRIN is classically known as an endothelial cell protein that sequesters eNOS and inhibits NO production. NOSTRIN contains a single polypeptide chain of 506-aa residues of 58 kDa with an N-terminal cdc15 domain and a C-terminal SH3 domain. NOSTRIN mRNA is abundant in highly vascularized tissues such as placenta, kidney, lung, and heart, and NOSTRIN protein is expressed in vascular endothelial cells.

Publikationen

Species	Application	Title
rat	WB	Redox Biol; Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro.; Authors - Dejuan Li
rat	WB	J Inflamm Res; TNF-α and IL-1β Promote Renal Podocyte Injury in T2DM Rats by Decreasing Glomerular VEGF/eNOS Expression Levels and Altering Hemodynamic Parameters; Authors - Jufang Wang
		Naunyn Schmiedebergs Arch Pharmacol; Thymoquinone mitigate ischemia-reperfusion-induced liver injury in rats: a pivotal role of nitric oxide signaling pathway.; Authors - Mohamed Abd-Elbaset
rat	WB	Mol Med Rep; Appropriate timing for hypothermic machine perfusion to preserve livers donated after circulatory death.; Authors - Xiaoyan Hu
rat	WB	Mol Med Rep; UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats.; Authors - Bingju Yan
human	WB	Mol Med Rep; Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II‑induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase.; Authors - Bingju Yan