NOTCH2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NOTCH2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28580-1-PBS

Synonyme

Notch homolog 2 (Drosophila), Notch 2, N2ICD, N2ECD, hN2

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  WB analysis using 28580-1-AP (same clone as 28580-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 28580-1-AP (NOTCH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28580-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 28580-1-AP (same clone as 28580-1-PBS)

  WB result of NOTCH2 antibody (28580-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and NOTCH2 knockout HEK-293T cells. This data was developed using the same antibody clone with 28580-1-PBS in a different storage buffer formulation.

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  IP experiment of U-87 MG using 28580-1-AP (same clone as 28580-1-PBS)

  IP result of anti-NOTCH2 (IP:28580-1-AP, 4ug; Detection:28580-1-AP 1:5000) with U-87 MG cells lysate 1960 ug. This data was developed using the same antibody clone with 28580-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 28580-1-AP (same clone as 28580-1-PBS)

  IP result of anti-NOTCH2 (IP:28580-1-AP, 4ug; Detection:28580-1-AP 1:5000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 28580-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 28580-1-AP (same clone as 28580-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28580-1-AP (NOTCH2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28580-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 28580-1-AP (same clone as 28580-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using NOTCH2 antibody (28580-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28580-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28580-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA NOTCH2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOTCH2 fusion protein Ag29413
• Vollständiger Name: Notch homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 265 kDa
• Beobachtetes Molekulargewicht: 110 kDa, 265 kDa
• GenBank-Zugangsnummer: NM_024408
• Gene symbol: NOTCH2
• Gene ID (NCBI): 4853
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).