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NOTCH2 Rekombinanter Antikörper

NOTCH2 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241162A2

Kat-Nr. : 84074-4-PBS

Synonyme

241162A2, hN2, N2ECD, N2ICD, Notch 2

Filter:
  • Western Blot (WB) analysis of various lysates using NOTCH2 Recombinant antibody (84074-4-RR)

    WB analysis using 84074-4-RR (same clone as 84074-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84074-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NOTCH2 Recombinant antibody (84074-4-RR)

    WB analysis using 84074-4-RR (same clone as 84074-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84074-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NOTCH2 Recombinant antibody (84074-4-RR)

    WB analysis of HEK-293 using 84074-4-RR (same clone as 84074-4-PBS)

    WB result of NOTCH2 antibody (84074-4-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NOTCH2 transfected HEK-293 cells. This data was developed using the same antibody clone with 84074-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84074-4-RR

    Affinity and Kinetic Characterization of 84074-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84074-4-RR against Human NOTCH2 were performed. The affinity constant is 1.86 nM.

Geprüfte Anwendungen

Produktinformation

84074-4-PBS bindet in WB, Indirect ELISA NOTCH2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen NOTCH2 fusion protein Ag29413
Vollständiger Name Notch homolog 2 (Drosophila)
Berechnetes Molekulargewicht 265 kDa
Beobachtetes Molekulargewicht 110 kDa, 265kDa
GenBank-ZugangsnummerNM_024408
Gene symbol NOTCH2
Gene ID (NCBI) 4853
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).