NOTCH2 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

NOTCH2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241162A2

Kat-Nr. : 84074-4-RR

Synonyme

241162A2, hN2, N2ECD, N2ICD, Notch 2

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  WB analysis using 84074-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84074-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84074-4-RR (NOTCH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 84074-4-RR

  WB result of NOTCH2 antibody (84074-4-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NOTCH2 transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 84074-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84074-4-RR against Human NOTCH2 were performed. The affinity constant is 1.86 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-87 MG-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84074-4-RR bindet in WB, ELISA NOTCH2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NOTCH2 fusion protein Ag29413
• Vollständiger Name: Notch homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 265 kDa
• Beobachtetes Molekulargewicht: 110 kDa, 265kDa
• GenBank-Zugangsnummer: NM_024408
• Gene symbol: NOTCH2
• Gene ID (NCBI): 4853
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOTCH2 (Neurogenic locus notch homolog protein 2) belongs to the NOTCH family.. In kidney, previous reports demonstrated that Notch1 and Notch2 were activated in mammalian nephrogenesis (PMID: 24526233). Notch2 is expressed in many cell types of most lineages in the hematolymphoid compartment and has specific roles in differentiation and function of various immune cells. Notch2 is required for development of splenic marginal zone B cells and regulates differentiation of dendritic cells (DCs) in the spleen. Notch2 appears to play some specific roles in the intestinal immunity, given that the fate of mast cells and a subset of DCs is regulated by Notch2 in the intestine. Notch2 also has important roles in helper T cell divergence from naïve CD4 T cells and activation of cytotoxic T cells (PMID: 22695918).