NOTCH3 Polyklonaler Antikörper

NOTCH3 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30540-1-AP

Synonyme

CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila), NOTCH3

---

Galerie der Validierungsdaten

• 

  WB analysis using 30540-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30540-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 30540-1-AP

  IP result of anti-NOTCH3(IP:30540-1-AP, 4ug; Detection:30540-1-AP 1:1000) with HeLa cells lysate 1320 ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HeLa-Zellen, Mauslungengewebe, U2OS-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30540-1-AP bindet in WB, IP, ELISA NOTCH3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOTCH3 fusion protein Ag30242
• Vollständiger Name: Notch homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 244 kDa
• Beobachtetes Molekulargewicht: 270 kDa, 90 kDa
• GenBank-Zugangsnummer: NM_000435
• Gene symbol: NOTCH3
• Gene ID (NCBI): 4854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOTCH3 (Neurogenic locus notch homolog protein 3) is one of four mammalian Notch proteins, which act as signalling receptors to control cell fate in many developmental and adult tissue contexts (PMID: 32210034). Notch is a transmembrane, developmental signalling receptor, which plays many crucial roles in developmental patterning, cell fate decisions, regulation of cell survival and proliferation (PMID:30246579). Genetic studies have shown that Notch3 gene knockouts are viable and have limited developmental defects, focussed mostly on defects in the arterial smooth muscle cell lineage. Notch3, in collaboration with other Notch proteins, is involved in stem cell regulation in different tissues in stem cell regulation in different tissues, and it also controls the plasticity of the vascular smooth muscle phenotype involved in arterial vessel remodelling. Overexpression, gene amplification and mis-activation of Notch3 are associated with different cancers, in particular triple negative breast cancer and ovarian cancer (PMID: 32210034).