NOTCH3 Polyklonaler Antikörper

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• KD/KO Validated

NOTCH3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55114-1-PBS

Synonyme

Notch homolog 3 (Drosophila), Notch 3, CASIL, CADASIL

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  WB analysis using 55114-1-AP (same clone as 55114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55114-1-AP (same clone as 55114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 55114-1-AP (same clone as 55114-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 55114-1-AP (same clone as 55114-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 55114-1-AP (same clone as 55114-1-PBS)

  IP result of anti-NOTCH3 (IP:55114-1-AP, 4ug; Detection:55114-1-AP 1:800) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 55114-1-AP (same clone as 55114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 55114-1-AP (same clone as 55114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55114-1-AP (same clone as 55114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 55114-1-AP (same clone as 55114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 55114-1-AP (same clone as 55114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 55114-1-AP (same clone as 55114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55114-1-PBS bindet in WB, IHC, IP, Indirect ELISA NOTCH3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Notch homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 244 kDa
• Beobachtetes Molekulargewicht: 250-260 kDa, 100 kDa
• GenBank-Zugangsnummer: NM_000435
• Gene symbol: NOTCH3
• Gene ID (NCBI): 4854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).