NOTUM Monoklonaler Antikörper

NOTUM Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F6D5

Kat-Nr. : 66956-1-PBS

Synonyme

2F6D5, EC:3.1.1.98, hNOTUM, OK/SW-CL.30, Palmitoleoyl-protein carboxylesterase NOTUM

---

• 

  WB analysis using 66956-1-Ig (same clone as 66956-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66956-1-Ig (same clone as 66956-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66956-1-Ig (same clone as 66956-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of T-47D using 66956-1-Ig (same clone as 66956-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66956-1-Ig (NOTUM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 66956-1-Ig (same clone as 66956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66956-1-Ig (NOTUM antibody) at dilution of 1:5000 (under 10x lens).. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 66956-1-Ig (same clone as 66956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66956-1-Ig (NOTUM antibody) at dilution of 1:5000 (under 40x lens).. This data was developed using the same antibody clone with 66956-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50968-3

  Cytometric bead array standard curve of MP50968-3, NOTUM Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66956-2-PBS. Detection antibody: 66956-1-PBS. Standard:Ag6338. Range: 0.098-100 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

66956-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA NOTUM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NOTUM fusion protein Ag6338
• Vollständiger Name: notum pectinacetylesterase homolog (Drosophila)
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC060882
• Gene symbol: NOTUM
• Gene ID (NCBI): 147111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.