NOV/CCN3 Rekombinanter Antikörper

NOV/CCN3 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250054B2

Kat-Nr. : 85731-1-RR

Synonyme

CCN family member 3, Ccn3, Cellular communication network factor 3, Nephroblastoma-overexpressed gene protein homolog, Nov

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  WB analysis using 85731-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85731-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85731-1-RR

  Mouse brain tissue was subjected to SDS PAGE followed by western blot with 85731-1-RR (Nov antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A172 cells, Caco-2-Zellen, HEK-293T-Zellen, HeLa-Zellen, Maushirngewebe, Maus-Thymusgewebe, Ratten-Thymusgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85731-1-RR bindet in WB, ELISA NOV/CCN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NOV/CCN3 fusion protein Eg2856
• Vollständiger Name: nephroblastoma overexpressed gene
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: NM_010930.4
• Gene symbol: Nov
• Gene ID (NCBI): 18133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCN family member 3 (CCN3), also known as Nephroblastoma overexpressed (NOV) protein, is a member of the CCN protein family (PMID: 1309586). CCN3 is predominantly expressed in the cytoplasm, nucleus, and extracellular space. As a subset of extracellular matrix proteins, it can directly bind to the integrin receptor family on the cell surface and participate in signal transduction between the cell surface and the extracellular matrix structure (PMID: 37378812). CCN3 exhibits anti-proliferative activity in normal cells and most tumor cells (PMID: 17340618). CCN3 co-localizes with connexin 43 (C×43) in the plasma membrane, and these two proteins physically interact to inhibit cell growth (PMID: 15213231). CCN3 can induce myofibroblast differentiation through the Notch pathway (PMID: 12050162). CCN can promote adhesion of endothelial cells, vascular smooth muscle cells, and fibroblasts through heparan sulfate proteoglycans (HSPG) (PMID: 30158025).