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NOX1 Rekombinanter Antikörper

NOX1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241935E7

Kat-Nr. : 84700-1-PBS

Synonyme

MOX1, MOX 1, Mitogenic oxidase 1, GP91 2, EC:1.6.3.-

Filter:
  • Western Blot (WB) analysis of various lysates using NOX1 Recombinant antibody (84700-1-RR)

    WB analysis using 84700-1-RR (same clone as 84700-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84700-1-RR (NOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84700-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84700-1-RR

    Affinity and Kinetic Characterization of 84700-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84700-1-RR against Human NOX1 were performed. The affinity constant is 0.364 nM.

Geprüfte Anwendungen

Produktinformation

84700-1-PBS bindet in WB, Indirect ELISA NOX1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen NOX1 fusion protein Ag12187
Vollständiger Name NADPH oxidase 1
Berechnetes Molekulargewicht 564 aa, 65 kDa
Beobachtetes Molekulargewicht 59 kDa
GenBank-ZugangsnummerBC075014
Gene symbol NOX1
Gene ID (NCBI) 27035
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NNOX1(NADPH oxidase 1) is also named as MOX1, NOH1 and belongs to the reactive oxygen species (ROS)-generating NADPH oxidase family. It plays a role in host defense, cell growth and differentiation, cell migration, and malignant transformation(PMID:20351171). It also can mediate oncogenic Ras-induced upregulation of VEGF and angiogenesis by activating Sp1 through Ras-ERK-dependent phosphorylation of Sp1(PMID:18454179). It has 3 isoforms produced by alternative splicing with the molecular weight of 65 kDa, 22 kDa and 59 kDa. The full length protein has two glycosylation sites. It always can be detected two bands of 63 kDa and 55 kDa in cells by western blot or another lower band of 45 kDa which is not likely due to alternative translation or splice variation, but as adjustment for the signal peptide and tag removal(PMID:17560373) or unspecifc(PMID:17460729).