NOX4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NOX4 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(338)

Rezensionen (1)

Kat-Nr. : 14347-1-AP

Synonyme

NOX4 Rabbit PolyAb, EC:1.6.3.1, Kidney oxidase 1, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase

---

• 

  WB analysis using 14347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14347-1-AP (NOX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 14347-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14347-1-AP (NOX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14347-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14347-1-AP (NOX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14347-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOX4 antibody (14347-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of A549 using 14347-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using NOX4 antibody (14347-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HeLa using 14347-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NOX4 (14347-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HEK-293-Zellen, HeLa-Zellen, JAR-Zellen, Mausnierengewebe, Mauslungengewebe, U-251-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 15 publications below
• WB: See 299 publications below
• IHC: See 53 publications below
• IF: See 36 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Produktinformation

14347-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA NOX4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOX4 fusion protein Ag5687
• Vollständiger Name: NADPH oxidase 4
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 58-67 kDa
• GenBank-Zugangsnummer: BC040105
• Gene symbol: NOX4
• Gene ID (NCBI): 50507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex. Studies have reported molecular masses of Nox4 protein by western blot analysis ranging from 55 to 80 kDa. The truncated NOX4 splice variant D (28 kDa) lacks the majority of the transmembrane domain and has been shown to produce higher levels of ROS and DNA damage compared to its prototype. NOX4D has previously been shown to localise to the nucleus and nucleolus in various cell types and is implicated in the generation of reactive oxygen species (ROS) and DNA damage. (PMID: 11728818, PMID: 29285262, PMID: 14670934)

Publikationen

Species	Application	Title
mouse	WB	Circulation; Megakaryocytic Leukemia 1 (MKL1) Bridges Epigenetic Activation of NADPH Oxidase in Macrophages to Cardiac Ischemia-Reperfusion Injury.; Authors - Yu Liming
		Nat Commun; Spatial oxidation of L-plastin downmodulates actin-based functions of tumor cells.; Authors - Emre Balta
mouse	WB	Aging Cell; Lysyl oxidase-like 2 inhibitor rescues D-galactose-induced skeletal muscle fibrosis.; Authors - Yongxin Wu
mouse	WB	Adv Healthc Mater; Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation; Authors - Ning Wang
human	WB,IF	Redox Biol; Silencing COX-2 blocks PDK1/TRAF4-induced AKT activation to inhibit fibrogenesis during skeletal muscle atrophy.; Authors - Hongtao Chen
rat	WB	Redox Biol; RND3 attenuates oxidative stress and vascular remodeling in spontaneously hypertensive rat via inhibiting ROCK1 signaling.; Authors - Nan Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

K (Verified Customer) (02-02-2022)

This antibody gave us really nice bands in 1:1000 dilution with 5% BSA+TBST.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela