NOX4 Monoklonaler Antikörper

NOX4 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

CloneNo.

4E5F1

Kat-Nr. : 67681-1-Ig

Synonyme

4E5F1, EC:1.6.3.1, Kidney oxidase 1, Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase

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  WB analysis using 67681-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67681-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-87 MG using 67681-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67681-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67681-1-Ig (NOX4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 67681-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67681-1-Ig (NOX4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 67681-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67681-1-Ig (NOX4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 67681-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using NOX4 antibody (67681-1-Ig, Clone: 4E5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HUVEC using 67681-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using NOX4 antibody (67681-1-Ig, Clone: 4E5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Produktinformation

67681-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA NOX4 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NOX4 fusion protein Ag6176
• Vollständiger Name: NADPH oxidase 4
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 58-67 kDa
• GenBank-Zugangsnummer: BC040105
• Gene symbol: NOX4
• Gene ID (NCBI): 50507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex. Studies have reported molecular masses of Nox4 protein by western blot analysis ranging from 55 to 80 kDa. The truncated NOX4 splice variant D (28 kDa) lacks the majority of the transmembrane domain and has been shown to produce higher levels of ROS and DNA damage compared to its prototype. NOX4D has previously been shown to localise to the nucleus and nucleolus in various cell types and is implicated in the generation of reactive oxygen species (ROS) and DNA damage (PMID: 11728818, PMID: 29285262, PMID: 14670934). Nox4 in cardiac myocytes is primarily expressed in mitochondria, and upregulation of Nox4 induced by hypertrophic stimuli elicits mitochondrial dysfunction and cardiac failure. In breast or ovarian tumor cells, mitochondrial Nox4 contributes to oncogenesis. In vascular endothelial cells, however, Nox4 is expressed in the endoplasmic reticulum (ER) and plays a specific role in redox-mediated ER signaling (PMID: 24259511).

Publikationen

Species	Application	Title
rat	WB	J Am Heart Assoc; Janus Kinase 2/Signal Transducer and Activator of Transcription 3/Cyclooxygenase 2 Signaling Pathway Mediates the Effect of Central Angiotensin II on the Elevation of Rostral Ventrolateral Medulla Prostaglandin E2-Induced Oxidative Stress in Hypertension; Authors - Jie-Fu Fan
human	WB	Biomedicines; Identification of NOX4 as a New Biomarker in Hepatocellular Carcinoma and Its Effect on Sorafenib Therapy; Authors - Hui-Zhou Li
human	WB	Exp Biol Med (Maywood); Triptolide decreases podocytes permeability by regulating TET2-mediated hydroxymethylation of ZO-1; Authors - Yue-Wen Tang
mouse	WB,IP,IF	J Pineal Res; Melatonin Alleviates Osteoarthritis by Regulating NADPH Oxidase 4-Induced Ferroptosis and Mitigating Mitochondrial Dysfunction; Authors - Qi Wang ; KD Validated
mouse	WB	Mech Ageing Dev; Deletion of Stimulator of Interferons Genes Aggravated Cardiac Dysfunction in Physiological Aged Mice; Authors - Diansa Gao
rat	WB,IF	Pflugers Arch; Placental ischemia-upregulated angiotensin II type 1 receptor in hypothalamic paraventricular nucleus contributes to hypertension in rat; Authors - Abdoulaye Issotina Zibrila