NOX4 Monoklonaler Antikörper

NOX4 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

4E5F1

Kat-Nr. : CL555-67681

Synonyme

Kidney oxidase 1, KOX, KOX 1, NADPH oxidase 4, NOX4, Renal NAD(P)H oxidase, RENOX

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Galerie der Validierungsdaten

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  FC experiment of HeLa using CL555-67681

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human NOX4 (CL555-67681, Clone:4E5F1) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-67681 bindet in FC (Intra) NOX4 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NOX4 fusion protein Ag6176
• Vollständiger Name: NADPH oxidase 4
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC040105
• Gene symbol: NOX4
• Gene ID (NCBI): 50507
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOX4 (NADPH oxidase 4) is a phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes with resultant antimicrobial activity and it has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex. Studies have reported molecular masses of Nox4 protein by western blot analysis ranging from 55 to 80 kDa. The truncated NOX4 splice variant D (28 kDa) lacks the majority of the transmembrane domain and has been shown to produce higher levels of ROS and DNA damage compared to its prototype. NOX4D has previously been shown to localise to the nucleus and nucleolus in various cell types and is implicated in the generation of reactive oxygen species (ROS) and DNA damage (PMID: 11728818, PMID: 29285262, PMID: 14670934). Nox4 in cardiac myocytes is primarily expressed in mitochondria, and upregulation of Nox4 induced by hypertrophic stimuli elicits mitochondrial dysfunction and cardiac failure. In breast or ovarian tumor cells, mitochondrial Nox4 contributes to oncogenesis. In vascular endothelial cells, however, Nox4 is expressed in the endoplasmic reticulum (ER) and plays a specific role in redox-mediated ER signaling (PMID: 24259511).