NOX5 Polyklonaler Antikörper

NOX5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, PLA, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 25350-1-AP

Synonyme

NADPH oxidase 5, NOX5, NOX5A, NOX5B

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Galerie der Validierungsdaten

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  WB analysis using 25350-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25350-1-AP (NOX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 25350-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25350-1-AP (NOX5 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 25350-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25350-1-AP (NOX5 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• CoIP: See 2 publications below

Produktinformation

25350-1-AP bindet in WB, PLA, IHC, CoIP, ELISA NOX5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NOX5 fusion protein Ag18673
• Vollständiger Name: NADPH oxidase, EF-hand calcium binding domain 5
• Berechnetes Molekulargewicht: 765 aa, 86 kDa
• Beobachtetes Molekulargewicht: 82-86 kDa
• GenBank-Zugangsnummer: BC125098
• Gene symbol: NOX5
• Gene ID (NCBI): 79400
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NOX5 (NADPH oxidase, EF-hand calcium binding domain 5) is an NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca(2+)-dependent manner. It plays an important role in PDGF-induced JAK/STAT activation and human aortic smooth muscle cell (HASMC) proliferation. NOX5 is the main source of superoxide and is implicated in human spermatozoa motility. This protein has 6 isoforms produced by alternative splicing, named NOX5-α, -β, -γ, -δ, -ε and -ζ with the molecular mass of 84, 82, 86, 85, 64 and 83 kDa (PMID: 22427510 and Uniprot).

Publikationen

Species	Application	Title
human	WB	Free Radic Biol Med; Chronic hypoxia of endothelial cells boosts HIF-1α-NLRP1 circuit in Alzheimer's disease; Authors - Eunyoung Jung
monkey,human	WB	Free Radic Biol Med; Nitric oxide and heme-NO stimulate superoxide production by NADPH oxidase 5.; Authors - Elizabeth A Sweeny
human	WB,CoIP	Front Cell Dev Biol; Functional interactions between NADPH oxidase 5 and actin; Authors - Samantha M Richter
human	WB	Ecotoxicol Environ Saf; Disruption of mitochondrial redox homeostasis as a mechanism of antimony-induced reactive oxygen species and cytotoxicity.; Authors - Liling Su
human	WB,CoIP,PLA	Biomed Pharmacother; Therapeutic effect of phycocyanin on acute liver oxidative damage caused by X-ray.; Authors - Qi Liu
human	WB	Biochem Biophys Res Commun; miR-485 suppresses inflammation and proliferation of mesangial cells in an in vitro model of diabetic nephropathy by targeting NOX5.; Authors - Jian Wu