Nucleoside phosphorylase Polyklonaler Antikörper

Nucleoside phosphorylase Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 18009-1-AP

Synonyme

PNP, EC:2.4.2.1, Inosine phosphorylase, Inosine-guanosine phosphorylase, PRO1837

---

• 

  WB analysis using 18009-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18009-1-AP (Nucleoside phosphorylase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18009-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18009-1-AP (Nucleoside phosphorylase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 18009-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 18009-1-AP (Nucleoside phosphorylase antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Raji-Zellen, U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

18009-1-AP bindet in WB, IF, ELISA Nucleoside phosphorylase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nucleoside phosphorylase fusion protein Ag12507
• Vollständiger Name: nucleoside phosphorylase
• Berechnetes Molekulargewicht: 289 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC106074
• Gene symbol: Purine nucleoside phosphorylase
• Gene ID (NCBI): 4860
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Purine nucleoside phosphorylases (PNP) is a ubiquitous enzyme of purine metabolism that functions in the salvage pathway, including even those of protozoan parasites, thus enabling the cells to utilize purine bases recovered from metabolized purine ribo- and deoxyribonucleosides to synthesize purine nucleotides (PMID: 11337031, 23332162). PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function. hPNP is widely distributed in various tissues and cells of the human body, with the highest activity in kidney, peripheral lymphocytes and granulocytes (PMID: 38701712).

Publikationen

Species	Application	Title
human	WB	J Hazard Mater; Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.; Authors - Ye Zhu
		J Biol Chem; Patient-derived organotypic tissue cultures as a platform to evaluate metabolic reprogramming in breast cancer patients; Authors - Teresa W -M Fan
canine	WB	Biologicals; CDC20 is a potential target gene to inhibit the tumorigenesis of MDCK cells; Authors - Zhenbin Liu
rat	WB,IF	Int Immunopharmacol; Spermidine attenuates monocrotaline-induced pulmonary arterial hypertension in rats by inhibiting purine metabolism and polyamine synthesis-associated vascular remodeling; Authors - Yu-Jing Chen
mouse,human	WB	Cell; De novo and salvage purine synthesis pathways across tissues and tumors; Authors - Diem H Tran
mouse,human	WB	J Ethnopharmacol; Diosmetin-7-O-β-D-glucopyranoside from Pogostemonis Herba alleviated SARS-CoV-2-induced pneumonia by reshaping macrophage polarization and limiting viral replication; Authors - Yun-Lu Xu