NPC1L1 Polyklonaler Antikörper

NPC1L1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28085-1-PBS

Synonyme

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  WB analysis using 28085-1-AP (same clone as 28085-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28085-1-AP (NPC1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28085-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 28085-1-AP (same clone as 28085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28085-1-AP (NPC1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28085-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 28085-1-AP (same clone as 28085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28085-1-AP (NPC1L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28085-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28085-1-PBS bindet in WB, IHC, Indirect ELISA NPC1L1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NPC1L1 fusion protein Ag26277
• Vollständiger Name: NPC1 (Niemann-Pick disease, type C1, gene)-like 1
• Berechnetes Molekulargewicht: 149 kDa
• Beobachtetes Molekulargewicht: 140-149 kDa
• GenBank-Zugangsnummer: NM_001101648
• Gene symbol: NPC1L1
• Gene ID (NCBI): 29881
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NPC1 like intracellular cholesterol transporter 1 (NPC1L1) is a multi-pass membrane protein which has 1332 amino acids and containing a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD). It is only found in the tubular membranes of primate hepatocytes and the brush-border membranes of the small intestine of mammals. PC1L1 is a key protein for intestinal absorption of cholesterol and plays an important role in cholesterol balance. Cholesterol contributes to cell proliferation, invasion and latency, and is essential for tumor formation and growth, so NPC1L1 plays a critical role in tumor development and spread (PMID: 36034854).