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NPC2 Rekombinanter Antikörper

NPC2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250911E11

Kat-Nr. : 86293-1-PBS

Synonyme

He1, Human epididymis-specific protein 1, Niemann-Pick disease type C2 protein

Filter:
  • Western Blot (WB) analysis of various lysates using NPC2 Recombinant antibody (86293-1-RR)

    WB analysis using 86293-1-RR (same clone as 86293-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86293-1-RR (NPC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86293-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NPC2 Recombinant antibody (86293-1-RR)

    WB analysis using 86293-1-RR (same clone as 86293-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86293-1-RR (NPC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86293-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NPC2 Recombinant antibody (86293-1-RR)

    IF Staining of HeLa using 86293-1-RR (same clone as 86293-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NPC2 antibody (86293-1-RR, Clone: 250911E11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86293-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86293-1-PBS bindet in WB, IF/ICC, Indirect ELISA NPC2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen NPC2 fusion protein Ag13719
Vollständiger Name Niemann-Pick disease, type C2
Berechnetes Molekulargewicht 151 aa, 17 kDa
Beobachtetes Molekulargewicht17-21 kDa
GenBank-ZugangsnummerBC002532
Gene symbol NPC2
Gene ID (NCBI) 10577
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Niemann-Pick Type C (NPC) disease is a lysosomal storage disorder characterized by accumulation of unesterified cholesterol and other lipids in the endolysosomal system. NPC disease results from a defect in either of two distinct cholesterol-binding proteins: a transmembrane protein, NPC1, and a small soluble protein, NPC2. NPC1 or NPC2 deficiency models showed that the function of these two proteins within lysosomes are linked closely. NPC2 is also named human epididymis-specific protein 1 (HE1), defects of which are the cause of Niemann-Pick disease type C2, characterized as a lysosomal storage disorder that affects the viscera and the central nervous system. Recent finding suggests that NPC2 may serve as a novel intracrine/autocrine factor that controls adipocyte differentiation and function as well as potential therapeutic target for the treatment of type 2 diabetes and related metabolic disorders.