NPEPPS Rekombinanter Antikörper

NPEPPS Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241502G8

Kat-Nr. : 84230-2-PBS

Synonyme

241502G8, AAP S, MP100, PSA

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  WB analysis using 84230-2-RR (same clone as 84230-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84230-2-RR (NPEPPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84230-2-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 84230-2-RR (same clone as 84230-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using NPEPPS antibody (84230-2-RR, Clone: 241502G8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84230-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84230-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84230-2-PBS against Human NPEPPS was performed. The affinity constant is below 1 pM.

• 

  Affinity and Kinetic Characterization of 84230-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84230-2-RR against Human NPEPPS were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84230-2-PBS bindet in WB, IF/ICC, Indirect ELISA NPEPPS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NPEPPS fusion protein Ag36035
• Vollständiger Name: aminopeptidase puromycin sensitive
• Beobachtetes Molekulargewicht: 103 kDa
• GenBank-Zugangsnummer: BC065294
• Gene symbol: NPEPPS
• Gene ID (NCBI): 9520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NPEPPS, also known as PSA, is a member of the oxytocinase subfamily of the M1 aminopeptidase family. NPEPPS encodes puromycin-sensitive aminopeptidase, a zinc metallopeptidase that hydrolyzes amino acids from the N-terminus of its substrate. The protein has 2 isoforms and is located in the cytoplasm.