NPEPPS Rekombinanter Antikörper

NPEPPS Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241502G8

Kat-Nr. : 84230-2-RR

Synonyme

PSA, MP100, AAP S, 241502G8

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  WB analysis using 84230-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84230-2-RR (NPEPPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 84230-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using NPEPPS antibody (84230-2-RR, Clone: 241502G8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84230-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84230-2-RR against Human NPEPPS were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293T-Zellen, MDCK-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84230-2-RR bindet in WB, IF/ICC, ELISA NPEPPS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NPEPPS fusion protein Ag36035
• Vollständiger Name: aminopeptidase puromycin sensitive
• Beobachtetes Molekulargewicht: 103 kDa
• GenBank-Zugangsnummer: BC065294
• Gene symbol: NPEPPS
• Gene ID (NCBI): 9520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NPEPPS, also known as PSA, is a member of the oxytocinase subfamily of the M1 aminopeptidase family. NPEPPS encodes puromycin-sensitive aminopeptidase, a zinc metallopeptidase that hydrolyzes amino acids from the N-terminus of its substrate. The protein has 2 isoforms and is located in the cytoplasm.