NPHP3 Polyklonaler Antikörper

NPHP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Rezensionen (1)

Kat-Nr. : 22026-1-AP

Synonyme

Nephrocystin-3, Nephrocystin 3, MKS7, KIAA2000

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  WB analysis using 22026-1-AP

  mouse testis lysates were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 22026-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22026-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 22026-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22026-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 22026-1-AP

  IP result of anti-NPHP3 (IP:22026-1-AP, 4ug; Detection:22026-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IHC staining of human heart using 22026-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22026-1-AP (NPHP3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 22026-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22026-1-AP (NPHP3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE1 cells using 22026-1-AP

  IF result (the base and the lower half of cilia stain) of anti-NPHP3 (22026-1-AP, 1:50) with serum-starved hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.

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  IF Staining of MDCK using 22026-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 22026-1-AP (NPHP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HEK-293-Zellen, HepG2-Zellen, humanes Hodengewebe, Mauslebergewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

22026-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NPHP3 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NPHP3 fusion protein Ag16888
• Vollständiger Name: nephronophthisis 3 (adolescent)
• Berechnetes Molekulargewicht: 1330 aa, 151 kDa
• Beobachtetes Molekulargewicht: 145-151 kDa
• GenBank-Zugangsnummer: BC068082
• Gene symbol: NPHP3
• Gene ID (NCBI): 27031
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Nat Methods; Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.; Authors - Su Steven S
human	IF	Am J Hum Genet; ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition.; Authors - Sumaya Alkanderi
human	IF	Hum Mol Genet; Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelial cells.; Authors - Nele Schwarz
mouse	IF	Int J Mol Sci; Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses.; Authors - Shweta Suiwal
mouse	IF	Mol Biol Cell; Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia; Authors - Vivek Reddy Palicharla
		Methods Cell Biol; Single-molecule imaging in the primary cilium; Authors - Lucien E Weiss

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lelongt (Verified Customer) (12-10-2021)

Immunofluorescence is observed in mice cilia but is not restricted to the inversin compartment. Dilution has to be adjusted for WB since there are few bands.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1/100 immunofluoresecence mice. 1/1000 WB dog
• Cell Tissue Type: mouce parrafin sections (immunoflorescence). MDCK cells (Western blot)