NPHP4 Polyklonaler Antikörper

NPHP4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 13812-1-AP

Synonyme

SLSN4, Nephroretinin, nephronophthisis 4, Nephrocystin-4, Nephrocystin 4

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  WB analysis of mouse brain using 13812-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13812-1-AP (NPHP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13812-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13812-1-AP (NPHP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 13812-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13812-1-AP (NPHP4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13812-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13812-1-AP (NPHP4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of hTERT-RPE1 cells using 13812-1-AP

  IF result from Dr. Corbit, Kevin. anti-NPHP4 (13812-1-AP) marks the ciliary axoneme, basal bodies, and the transition zone of Human hTERT-RPE1 cells.

• 

  IF Staining of MDCK using 13812-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 13812-1-AP (NPHP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 11 publications below

Produktinformation

13812-1-AP bindet in WB, IHC, IF/ICC, ELISA NPHP4 und zeigt Reaktivität mit human, Hund, Maus

• Getestete Reaktivität: human, Hund, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NPHP4 fusion protein Ag4875
• Vollständiger Name: nephronophthisis 4
• Berechnetes Molekulargewicht: 1426 aa, 158 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC040520
• Gene symbol: NPHP4
• Gene ID (NCBI): 261734
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NPHP4, also named as KIAA0673, is a cilia-associated protein which involved in the organization of apical junctions in kidney cells together with NPHP1 and RPGRIP1L/NPHP8. It is necessary for normal photoreceptor ribbon synapse maintenance and outer segment formation, and for sperm development.

Publikationen

Species	Application	Title
human,mouse	IF	Nat Genet; A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.; Authors - Garcia-Gonzalo Francesc R FR
mouse	IF	Nat Cell Biol; Lineage specificity of primary cilia in the mouse embryo.; Authors - Fiona K Bangs
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
human	IF	Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
mouse	IF	J Cell Biol; The small GTPase RSG1 controls a final step in primary cilia initiation.; Authors - Stephanie O Agbu
human	IF	Genetics; Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the Atypical MAP Kinase MAPK15 in Caenorhabditis elegans and Human Cells.; Authors - Anna Kazatskaya