NPHP5/IQCB1 Polyklonaler Antikörper

NPHP5/IQCB1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15747-1-AP

Synonyme

IQCB1, NPHP5, IQ calmodulin-binding motif-containing protein 1, IQ motif containing B1, IQCB 1

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  WB analysis using 15747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15747-1-AP (NPHP5,IQCB1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15747-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15747-1-AP (NPHP5/IQCB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15747-1-AP

  IP result of anti-NPHP5/IQCB1 (IP:15747-1-AP, 4ug; Detection:15747-1-AP 1:1000) with HEK-293 cells lysate 1280 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

15747-1-AP bindet in WB, IF, IP, ELISA NPHP5/IQCB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NPHP5/IQCB1 fusion protein Ag8203
• Vollständiger Name: IQ motif containing B1
• Berechnetes Molekulargewicht: 598 aa, 69 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC005806
• Gene symbol: IQCB1
• Gene ID (NCBI): 9657
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

IQCB1, also known as NPHP5, is a nephrocystin protein that interacts with calmodulin and the retinitis pigmentosa GTPase regulator protein. It has a central coiled-coil region and two calmodulin-binding IQ domains. Localized to the primary cilia of renal epithelial cells and connecting cilia of photoreceptor cells, IQCB1 is thought to play a role in ciliary function. Mutations in this gene result in Senior-Loken syndrome type 5, a juvenile disorder characterized by defects in the waste filtering system of the kidney, as well as retinal degradation.

Publikationen

Species	Application	Title
human	IF	Hum Mol Genet; Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis.; Authors - Barbelanne Marine M
human		Mol Biol Cell; Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone.; Authors - Antonia Wiegering