NPL Rekombinanter Antikörper

NPL Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243037E2

Kat-Nr. : 85608-3-RR

Synonyme

c112, C1orf13, EC:4.1.3.3, N acetylneuraminate lyase, N-acetylneuraminate lyase

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  WB analysis using 85608-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85608-3-RR (NPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 85608-3-RR

  rat brain tissue were subjected to SDS PAGE followed by western blot with 85608-3-RR (NPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85608-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85608-3-RR against Human NPL were performed. The affinity constant is 4.26 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maushirngewebe, Rattenhirngewebe, Rattennierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85608-3-RR bindet in WB, ELISA NPL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NPL fusion protein Ag10137
• Vollständiger Name: N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)
• Berechnetes Molekulargewicht: 27 kDa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC058003
• Gene symbol: NPL
• Gene ID (NCBI): 80896
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The N-acetylneuraminate lyase(NPL) from human (hNAL) which consists of 320 amino acids, has a molecular mass of 35.2 kDa and it catalyses the reversible aldol reaction of sialic acid, and has been extensively used in the synthesis of sialic acids and its analogues(PMID:19057931). The enzyme is widely distributed and are present in numerous pro- and eukaryotic cells, in which they are localized only in the cytosol(PMID:12453637). It belongs to the DHDPS family and has 5 isoforms produced by alternative splicing.