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NPM3 Rekombinanter Antikörper

NPM3 Rekombinant Antikörper für IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242863G12

Kat-Nr. : 85156-5-PBS

Synonyme

242863G12, nucleophosmin/nucleoplasmin, 3, Nucleoplasmin 3, Nucleoplasmin-3, PORMIN

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NPM3 Recombinant antibody (85156-5-RR)

    IF Staining of HeLa using 85156-5-RR (same clone as 85156-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NPM3 antibody (85156-5-RR, Clone: 242863G12 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85156-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85156-5-RR

    Affinity and Kinetic Characterization of 85156-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85156-5-RR against Human NPM3 were performed. The affinity constant is 1.98 nM.

Geprüfte Anwendungen

Produktinformation

85156-5-PBS bindet in IF/ICC, Indirect ELISA NPM3 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen NPM3 fusion protein Ag2564
Vollständiger Name nucleophosmin/nucleoplasmin, 3
Berechnetes Molekulargewicht 178 aa, 19 kDa
GenBank-ZugangsnummerBC054868
Gene symbol NPM3
Gene ID (NCBI) 10360
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NPM3, a member of the nucleophosmin/ nucleoplasmin family, lacks intrinsic histone chaperone activity, inhibits histone assembly activity of NPM1 in vitro, and dramatically enhances transcription in a cellular system(PMID: 20073534). Human NPM3 is an abundant and widely expressed protein with primarily nuclear localization. The NPM3 protein migrated in SDS-PAGE gels as a doublet with apparent molecular masses of 25 and 27 kDa, which are larger than the 20.5 kDa mass predicted by the deduced amino acid sequence including the HA tag (PMID: 11722795).