NPPA Polyklonaler Antikörper

NPPA Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27426-1-PBS

Synonyme

ANP, PND, ANF, ATFB6, Atrial natriuretic factor

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  WB analysis using 27426-1-AP (same clone as 27426-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 27426-1-AP (same clone as 27426-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 27426-1-AP (NPPA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27426-1-AP (same clone as 27426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 27426-1-AP (same clone as 27426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 27426-1-AP (same clone as 27426-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27426-1-AP (NPPA antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 27426-1-AP (same clone as 27426-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using NPPA antibody (27426-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 27426-1-AP (same clone as 27426-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NPPA antibody (27426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27426-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27426-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NPPA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NPPA fusion protein Ag26692
• Vollständiger Name: natriuretic peptide precursor A
• Berechnetes Molekulargewicht: 153 aa, 17 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC005893
• Gene symbol: NPPA
• Gene ID (NCBI): 4878
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Natriuretic peptide precursor-A (NPPA) is an early and specific marker for functional myocardium of the embryonic heart. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. NPPA is expressed primarily in the heart, where the expression level is higher in atria than ventricles. Low levels of ANP expression have been detected in other tissues, including the lung, aorta, brain, adrenal gland, kidney and uterus.