BNP Polyklonaler Antikörper

BNP Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 13299-1-AP

Synonyme

BNP, BNP(1 32), Natriuretic peptides B, NPPB

---

Galerie der Validierungsdaten

• 

  IHC staining of mouse brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13299-1-AP (BNP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13299-1-AP (BNP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13299-1-AP (BNP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 13299-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 13299-1-AP (BNP Antibody) at dilution of 1:50.

• 

  IF Staining of rat brain using 13299-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using BNP antibody (13299-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 13299-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using BNP antibody (13299-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Herzgewebe, humanes Hirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe, HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

13299-1-AP bindet in IHC, IF, ELISA BNP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BNP fusion protein Ag4039
• Vollständiger Name: natriuretic peptide precursor B
• Berechnetes Molekulargewicht: 134 aa, 15 kDa
• GenBank-Zugangsnummer: BC025785
• Gene symbol: NPPB
• Gene ID (NCBI): 4879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NPPB, also known as B-type natriuretic peptide (BNP), is a cardiac hormone that is secreted mainly in the ventricles in response to increased wall stress. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. BNP is a marker of systolic and diastolic dysfunction and a strong predictor of mortality in heart failure patients.

Publikationen

Species	Application	Title
rat		Front Cardiovasc Med; Effects of Excessive Activation of N-methyl-D-aspartic Acid Receptors in Neonatal Cardiac Mitochondrial Dysfunction Induced by Intrauterine Hypoxia.; Authors - Yang Liu
		Pharm Biol; Epigallocatechin gallate (EGCG) attenuates myocardial hypertrophy and fibrosis induced by transverse aortic constriction via inhibiting the Akt/mTOR pathway.; Authors - Yue Cui
		Int Heart J; Phenotype and Functional Analyses in a Transgenic Mouse Model of Left Ventricular Noncompaction Caused by a DTNA Mutation.; Authors - Qing Cao
rat		Cell Regen; Fabrication of SA/Gel/C scaffold with 3D bioprinting to generate micro-nano porosity structure for skin wound healing: a detailed animal in vivo study.; Authors - Changmei Niu
	IHC	Front Pharmacol; Administration of USP7 inhibitor P22077 inhibited cardiac hypertrophy and remodeling in Ang II-induced hypertensive mice; Authors - Yu-Hui Gu
rat		Mol Med Rep; G9a inhibition promotes the formation of pacemaker-like cells by reducing the enrichment of H3K9me2 in the HCN4 promoter region; Authors - Pei Xu