NPTX2 Rekombinanter Antikörper

NPTX2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250809A7

Kat-Nr. : 86203-1-RR

Synonyme

Neuronal pentraxin II, Neuronal pentraxin-2, NP2, NP-II

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  WB analysis using 86203-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86203-1-RR (NPTX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 86203-1-RR

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 86203-1-RR (NPTX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 86203-1-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 86203-1-RR (NPTX2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86203-1-RR bindet in WB, IHC, ELISA NPTX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NPTX2 fusion protein Ag33808
• Vollständiger Name: neuronal pentraxin II
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47-50 kDa
• GenBank-Zugangsnummer: BC009924
• Gene symbol: NPTX2
• Gene ID (NCBI): 4885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)