NQO1 Rekombinanter Antikörper

NQO1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251713C3

Kat-Nr. : 86693-2-RR

Synonyme

Azoreductase, DHQU, DIA 4, DIA4, DT diaphorase

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  WB analysis using 86693-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86693-2-RR (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86693-2-RR

  HSC-T6 cell lysates were subjected to SDS PAGE followed by western blot with 86693-2-RR (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86693-2-RR

  K-562 cell lysates were subjected to SDS PAGE followed by western blot with 86693-2-RR (NQO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86693-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86693-2-RR against Human NQO1 were performed. The affinity constant is 1.80 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, K-562-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86693-2-RR bindet in WB, ELISA NQO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: NQO1 fusion protein Ag2009
• Vollständiger Name: NAD(P)H dehydrogenase, quinone 1
• Berechnetes Molekulargewicht: 274 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC007659
• Gene symbol: NQO1
• Gene ID (NCBI): 1728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NQO1, also named as DIA4, NMOR1, DTD and QR1, belongs to the NAD(P)H dehydrogenase (quinone) family. This enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is known to be involved in benzene metabolism. In human studies of ozone exposure, polymorphisms in oxidative stress genes (NQO1, GSTM1, GSTP1) modify respiratory symptoms, lung function, biomarkers and risk of asthma. (PMID:18511640, PMID:18848868)