NQO2 Polyklonaler Antikörper

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• KD/KO Validated

NQO2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 15767-1-AP

Synonyme

NMOR2, EC:1.10.5.1

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  WB analysis using 15767-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15767-1-AP (NQO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15767-1-AP

  IP result of anti-NQO2 (IP:15767-1-AP, 3ug; Detection:15767-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of mouse testis using 15767-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15767-1-AP (NQO2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15767-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15767-1-AP (NQO2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15767-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NQO2 antibody (15767-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below

Produktinformation

15767-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA NQO2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NQO2 fusion protein Ag8403
• Vollständiger Name: NAD(P)H dehydrogenase, quinone 2
• Berechnetes Molekulargewicht: 231 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC006096
• Gene symbol: NQO2
• Gene ID (NCBI): 4835
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.

Publikationen

Species	Application	Title
human	WB	Antioxidants (Basel); Apigenin and Luteolin Regulate Autophagy by Targeting NRH-Quinone Oxidoreductase 2 in Liver Cells.; Authors - Elzbieta Janda
human	WB	J Exp Clin Cancer Res; PRX1 knockdown potentiates vitamin K3 toxicity in cancer cells: a potential new therapeutic perspective for an old drug.; Authors - Tiantian He
rat	WB	J Proteome Res; Proteomic and Phosphoproteomic Analysis of Right Ventricular Hypertrophy in the Pulmonary Hypertension Rat Model; Authors - Ang Luo
rat	WB	Cell Physiol Biochem; Downregulation of quinone reductase 2 attenuates vascular smooth muscle cells proliferation and neointimal formation in balloon injured rat carotid artery.; Authors - Zhang Xi-wen XW ; KD Validated
human	WB	Life Sci Alliance; Tanshinone functions as a coenzyme that confers gain of function of NQO1 to suppress ferroptosis; Authors - Tian-Xiang Wang
human,mouse	WB	J Appl Toxicol; Realgar-induced nephrotoxicity via ferroptosis in mice; Authors - Sheng Zhang