NQO2 Polyklonaler Antikörper

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• KD/KO Validated

NQO2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15767-1-PBS

Synonyme

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  WB analysis using 15767-1-AP (same clone as 15767-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15767-1-AP (NQO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15767-1-AP (same clone as 15767-1-PBS)

  IP result of anti-NQO2 (IP:15767-1-AP, 3ug; Detection:15767-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15767-1-AP (same clone as 15767-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15767-1-AP (NQO2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15767-1-AP (same clone as 15767-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15767-1-AP (NQO2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15767-1-AP (same clone as 15767-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NQO2 antibody (15767-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15767-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15767-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA NQO2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NQO2 fusion protein Ag8403
• Vollständiger Name: NAD(P)H dehydrogenase, quinone 2
• Berechnetes Molekulargewicht: 231 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC006096
• Gene symbol: NQO2
• Gene ID (NCBI): 4835
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.