NR1D1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NR1D1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14506-1-PBS

Synonyme

ear 1, EAR1, EAR-1, HREV, Rev erbA alpha

---

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 cells using 14506-1-AP (same clone as 14506-1-PBS)

  WB result of anti-NR1D1 (14506-1-AP,1:1000) with A549 cell by Dr. Toryn Poolman. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 cells using 14506-1-AP (same clone as 14506-1-PBS)

  WB result of anti-NR1D1 (14506-1-AP,1:500) with A549 treated with Cycloheximide by Dr. Toryn Poolman. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 14506-1-AP (same clone as 14506-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14506-1-AP (NR1D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 14506-1-AP (same clone as 14506-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14506-1-AP (NR1D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 14506-1-AP (same clone as 14506-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 14506-1-AP (NR1D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

14506-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA NR1D1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NR1D1 fusion protein Ag5964
• Vollständiger Name: nuclear receptor subfamily 1, group D, member 1
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC056148
• Gene symbol: NR1D1
• Gene ID (NCBI): 9572
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.