NR1D2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NR1D2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 13906-1-AP

Synonyme

BD73, EAR 1R, EAR-1R, Rev erb beta, Rev-erb alpha-related receptor

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  WB analysis of Jurkat using 13906-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13906-1-AP (NR1D2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13906-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13906-1-AP (NR1D2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13906-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13906-1-AP (NR1D2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 13906-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using NR1D2 antibody (13906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Produktinformation

13906-1-AP bindet in WB, IHC, IF/ICC, ELISA NR1D2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NR1D2 fusion protein Ag4881
• Vollständiger Name: nuclear receptor subfamily 1, group D, member 2
• Berechnetes Molekulargewicht: 579 aa, 65 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC045613
• Gene symbol: NR1D2
• Gene ID (NCBI): 9975
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Sci Rep; The functional and predictive roles of miR-210 in cryptorchidism.; Authors - Zhengzheng Duan
mouse	WB	Am J Physiol Heart Circ Physiol; Differential effects of REV-ERBα/β agonism on cardiac gene expression, metabolism, and contractile function in a mouse model of circadian disruption.; Authors - Sobuj Mia
human	WB	Xenobiotica; Identification of rhythmic human CYPs and their circadian regulators using synchronized hepatoma cells.; Authors - Min Chen
human	WB	Cell Death Dis; SR9009 inhibits lethal prostate cancer subtype 1 by regulating the LXRα/FOXM1 pathway independently of REV-ERBs; Authors - Hang Xu ; KD Validated
rat,human	WB	NPJ Biofilms Microbiomes; Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signaling; Authors - Shang Li
mouse	IHC	J Biomed Sci; Secretome from estrogen-responding human placenta-derived mesenchymal stem cells rescues ovarian function and circadian rhythm in mice with cyclophosphamide-induced primary ovarian insufficiency; Authors - Duy-Cuong Le