NR1H4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NR1H4 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(85)

Rezensionen (1)

Kat-Nr. : 25055-1-AP

Synonyme

BAR, Bile acid receptor, Farnesoid X activated receptor, Farnesoid X-activated receptor, Farnesol receptor HRR-1

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  WB analysis of HepG2 using 25055-1-AP

  WB result of NR1H4 antibody (25055-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and NR1H4 knockout HepG2 cells.

• 

  WB analysis using 25055-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25055-1-AP (NR1H4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat liver using 25055-1-AP

  IP result of anti-NR1H4 (IP:25055-1-AP, 4ug; Detection:25055-1-AP 1:2000) with rat liver tissue lysate 2600 ug.

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  IHC staining of human kidney using 25055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25055-1-AP (NR1H4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 25055-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25055-1-AP (NR1H4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen
• Erfolgreiche IP: Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 71 publications below
• IHC: See 16 publications below
• IF: See 19 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

25055-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA NR1H4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NR1H4 fusion protein Ag21878
• Vollständiger Name: nuclear receptor subfamily 1, group H, member 4
• Berechnetes Molekulargewicht: 486 aa, 56 kDa
• Beobachtetes Molekulargewicht: 48-56 kDa
• GenBank-Zugangsnummer: BC130573
• Gene symbol: NR1H4
• Gene ID (NCBI): 9971
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear Receptor subfamily 1, group H, member 4 (NR1H4, also known as FXR) is a receptor for bile acids and has an important role in regulating energy metabolism in liver, muscle and adipose tissues in humans and animals. NR1H4 is highly expressed in liver and has a role in lipid metabolism, whereas none of the other protein-coding genes in the region has known biological links with cholesterol, further supporting a role for NR1H4 in regulation of cholesterol levels. NR1H4 have five isoforms, each isoform has a different expressional level and transcriptional activity depending on its location within specific tissues. (PMID: 14733360, PMID: 30787420)

Publikationen

Species	Application	Title
mouse	WB	Cell Host Microbe; Dietary fiber alleviates alcoholic liver injury via Bacteroides acidifaciens and subsequent ammonia detoxification; Authors - Haiyuan Shen ; KD Validated
human	CoIP	Cell Host Microbe; A gut microbiota-bile acid axis promotes intestinal homeostasis upon aspirin-mediated damage; Authors - Ting Li
mouse	WB,IF	Sci Total Environ; Bisphenol A induced hepatic steatosis by disturbing bile acid metabolism and FXR/TGR5 signaling pathways via remodeling the gut microbiota in CD-1 mice; Authors - Ting Hong
rat	WB,IF	Phytomedicine; Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis; Authors - Daofei Song
rat	WB	Phytomedicine; Jiang-Tang-San-Huang pill alleviates type 2 diabetes mellitus through modulating the gut microbiota and bile acids metabolism; Authors - Dina Tawulie
mouse,human	WB,IHC	Biomater Sci; Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis; Authors - Dickson Kofi Wiredu Ocansey

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Bryce (Verified Customer) (04-16-2021)

Antibody did not work for mouse kidney homogenates. There was no observable difference between kidneys from wild type and knockout mice. Genotypes of blotted samples were confirmed by RT-qPCR.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse kidney

NR1H4 Antibody Western Blot validation (1:1000 dilution) in mouse kidney (Cat no:25055-1-AP)