CAR/NR1I3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CAR/NR1I3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (3)

Kat-Nr. : 21042-1-AP

Synonyme

CAR, Constitutive androstane receptor, NR1I3, CAR BETA, CAR1

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  WB analysis of HeLa using 21042-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21042-1-AP (CAR/NR1I3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21042-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21042-1-AP (NR1I3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21042-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21042-1-AP (NR1I3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 21042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21042-1-AP (CAR/NR1I3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 21042-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21042-1-AP (CAR/NR1I3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 21042-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 21042-1-AP (NR1I3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 21042-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 21042-1-AP (NR1I3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below

Produktinformation

21042-1-AP bindet in WB, IHC, ELISA CAR/NR1I3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CAR/NR1I3 fusion protein Ag15378
• Vollständiger Name: nuclear receptor subfamily 1, group I, member 3
• Berechnetes Molekulargewicht: 352 aa, 40 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC069651
• Gene symbol: NR1I3
• Gene ID (NCBI): 9970
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The nuclear hormone receptor superfamily is a large group of related transcription factors which includes members that bind a diverse array of ligands, including steroids, retinoic acid, thyroid hormone, and vitamin D. Nuclear hormone receptors contain a DNA-binding domain (DBD) and a ligand-binding domain [PMID: 10221899]. Nuclear receptor subfamily 1 group I member 3 (NR1I3; Constitutive androstane receptor: CAR), binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. It is expressed primarily in liver and regulates the expression of genes involved in xenobiotic metabolism as well as hormone, energy, and lipid homeostasis [PMID:22896671].

Publikationen

Species	Application	Title
human	WB	Biochem Pharmacol; Constitutive androstane receptor weakens the induction of panaxytriol on CYP3A4 by repressing the activation of pregnane X receptor.; Authors - Qingqing Hu ; KD Validated
human	WB	Front Pharmacol; Oleanolic Acid and Ursolic Acid Induce UGT1A1 Expression in HepG2 Cells by Activating PXR Rather Than CAR.; Authors - Na Yao ; KD Validated
human	WB	Xenobiotica; A high-throughput cell-based gaussia luciferase reporter assay for measurement of CYP1A1, CYP2B6, and CYP3A4 induction.; Authors - Han Li
human	WB	J Ethnopharmacol; Panaxytriol upregulates CYP3A4 expression through the interaction between nuclear regulators and DNA response elements; Authors - Jingdi Yan ; KD Validated
mouse,human	WB	PLoS Pathog; Lactobacillus gasseri ATCC33323 affects the intestinal mucosal barrier to ameliorate DSS-induced colitis through the NR1I3-mediated regulation of E-cadherin; Authors - Guanru Qian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

This antibody never lets me down. Purchased again for immunohistochemistry.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse liver tissue

FH

Kamal (Verified Customer) (07-05-2023)

Worked well when diluted in TBST (1:2000) for WB and 1:200 in 1X PBS for IFC.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 (WB) and 1:200 (IFC)
• Cell Tissue Type: Mouse Liver tissue

FH

Zehua (Verified Customer) (11-07-2019)

It works well for immunoprecipitation!

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2 cell

CAR/NR1I3 Antibody Western Blot,Immunoprecipitation, validation (1:1000 dilution) in HepG2 cell (Cat no:21042-1-AP)