NR2E3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NR2E3 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 14246-1-AP

Synonyme

ESCS, NR2E3, PNR, rd7, RNR, RP37

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Galerie der Validierungsdaten

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  WB analysis using 14246-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14246-1-AP (NR2E3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14246-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14246-1-AP (NR2E3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14246-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14246-1-AP (NR2E3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 14246-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14246-1-AP (NR2E3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14246-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14246-1-AP (NR2E3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 14246-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14246-1-AP (NR2E3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of photoreceptor progenitor cells using 14246-1-AP

  IF result of NR2E3 antibody (14246-1-AP, 1:50) with human photoreceptor progenitors cells.

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  FC experiment of photoreceptor progenitor cells using 14246-1-AP

  FC result of NR2E3 antibody (14246-1-AP, 1:100) with human photoreceptor progenitors cells. Blue, isotype control; Black=passage 2; Red=passage 4.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Retina-Gewebe, HepG2-Zellen, Ratten-Retina-Gewebe, Y79-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Photorezeptor-Vorläuferzellen
• Erfolgreiche Detektion in FC: Photorezeptor-Vorläuferzellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:100
• Durchflusszytometrie (FC): FC : ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

14246-1-AP bindet in WB, IHC, IF, FC, ELISA NR2E3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NR2E3 fusion protein Ag5503
• Vollständiger Name: nuclear receptor subfamily 2, group E, member 3
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: BC041421
• Gene symbol: NR2E3
• Gene ID (NCBI): 10002
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR2E3, also known as PNR, encodes a retinal nuclear receptor that is a ligand-dependent transcription factor. This protein is part of a large family of nuclear receptor transcription factors involved in signaling pathways. NR2E3 influences the development of photoreceptors and their differentiation into rod and cone types, and acts as a ranscriptional factor that is an activator of rod development and repressor of cone development [PMID:20725840]. It binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. [PMID:15689355]

Publikationen

Species	Application	Title
mouse	IHC	PLoS Genet; Distinct and Atypical Intrinsic and Extrinsic Cell Death Pathways between Photoreceptor Cell Types upon Specific Ablation of Ranbp2 in Cone Photoreceptors.; Authors - Cho Kyoung-In KI
mouse	WB	J Biol Chem; Differential Loss of Prolyl-Isomerase or Chaperone Activity of Ran-binding protein 2 (Ranbp2) Unveils Distinct Physiological Roles of Its Cyclophilin Domain in Proteostasis.; Authors - Kyoung-In Cho
human	WB	Sci Rep; Loss of NR2E3 represses AHR by LSD1 reprogramming, is associated with poor prognosis in liver cancer.; Authors - Tilak Khanal
mouse	WB	FASEB J; NR2E3 is a key component in p53 activation by regulating a long noncoding RNA DINO in acute liver injuries.; Authors - Tilak Khanal ; KO Validated
zebrafish	IHC	Am J Pathol; Dysregulation of Spliceosomes Complex Induces Retinitis Pigmentosa-Like Characteristics in sf3b4-Depleted Zebrafish; Authors - Zulvikar Syambani Ulhaq