NR2F6 Monoklonaler Antikörper

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• KD/KO Validated

NR2F6 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H2B8

Kat-Nr. : 60117-2-PBS

Synonyme

2H2B8, EAR 2, EAR2, EAR-2, ERBAL2

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  WB analysis of HeLa using 60117-2-Ig (same clone as 60117-2-PBS)

  WB result of NR2F6 antibody (60117-2-Ig, 1:1000) with si-Control and si-NR2F6 transfected HeLa cells. This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

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  WB analysis using 60117-2-Ig (same clone as 60117-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60117-2-Ig (NR2F6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60117-2-Ig (same clone as 60117-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60117-2-Ig (NR2F6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60117-2-Ig (same clone as 60117-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60117-2-Ig (NR2F6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60117-2-Ig (same clone as 60117-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60117-2-Ig(NR2F6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60117-2-Ig (same clone as 60117-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 60117-2-Ig(NR2F6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 60117-2-Ig (same clone as 60117-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60117-2-Ig (NR2F6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60117-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60117-2-PBS bindet in WB, IHC, Indirect ELISA NR2F6 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NR2F6 fusion protein Ag7614
• Vollständiger Name: nuclear receptor subfamily 2, group F, member 6
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC002669
• Gene symbol: NR2F6
• Gene ID (NCBI): 2063
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR2F6 belongs to NR2F subfamily members, which have their roles in the regulation of neurogenesis, organogenesis, and cellular differentiation during embryonic development (PMID:15875024). NR2F6 acts as transcription factor by binding to a TGACCT direct-repeat motif and has been established in controlling facets of central nervous system (CNS) (PMID: 12690040). It is a nuclear attenuator that directly interferes with DNA binding of NF-AT and, subsequently, transcriptional activity of the NF-AT-dependent IL-17 expression. Otherwise, NR2F6 negatively interfered with transcriptional cytokine responses and acted as a barrier against autoimmunity (PMID:18701084).