NR3C2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

NR3C2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (1)

Kat-Nr. : 21854-1-AP

Synonyme

MCR, Mineralocorticoid receptor, MLR, MR, NR3C2

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 21854-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21854-1-AP

  WB result of NR3C2 antibody (21854-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR3C2 transfected HEK-293 cells.

• 

  WB analysis using 21854-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 21854-1-AP

  IP Result of anti-NR3C2 (IP:21854-1-AP, 4ug; Detection:21854-1-AP 1:500) with HEK-293 cells lysate 3200ug.

• 

  IHC staining of human kidney using 21854-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21854-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 21854-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 21854-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 21854-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 21854-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• ChIP: See 3 publications below

Produktinformation

21854-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA NR3C2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NR3C2 fusion protein Ag16410
• Vollständiger Name: nuclear receptor subfamily 3, group C, member 2
• Berechnetes Molekulargewicht: 984 aa, 107 kDa
• Beobachtetes Molekulargewicht: 94-110 kDa
• GenBank-Zugangsnummer: BC111758
• Gene symbol: NR3C2
• Gene ID (NCBI): 4306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.

Publikationen

Species	Application	Title
rat	IHC	J Pineal Res; Melatonin prevents experimental central serous chorioretinopathy in rats.; Authors - Shanshan Yu
human	WB	EMBO Rep; High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction.; Authors - Yukang Yuan
rat	WB	Oncotarget; Impact of prenatal cold stress on placental physiology, inflammatory response, and apoptosis in rats.; Authors - Shuai Lian
rat	IHC,IF	Int Immunopharmacol; UUO induces lung fibrosis with macrophage-myofibroblast transition in rats.; Authors - Fan Yang
rat	WB	Behav Brain Res; Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.; Authors - Shuai Lian
rat	WB	Behav Brain Res; Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.; Authors - Shuai Lian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Paloma (Verified Customer) (06-01-2022)

Good performance in ChIP

• Applications: ChIP
• Cell Tissue Type: Immortalized keratinocytes