NR3C2 Polyklonaler Antikörper

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• KD/KO Validated

NR3C2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21854-1-PBS

Synonyme

MCR, MLR, MR

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  WB analysis of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)

  WB result of NR3C2 antibody (21854-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NR3C2 transfected HEK-293 cells. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

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  WB analysis using 21854-1-AP (same clone as 21854-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21854-1-AP (NR3C2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)

  IP result of anti-NR3C2 (IP:21854-1-AP, 4ug; Detection:21854-1-AP 1:500) with HEK-293 cells lysate 3200ug. This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 21854-1-AP (same clone as 21854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 21854-1-AP (same clone as 21854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 21854-1-AP (same clone as 21854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 21854-1-AP (same clone as 21854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 21854-1-AP (same clone as 21854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21854-1-AP (NR3C2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 21854-1-AP (same clone as 21854-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 21854-1-AP (same clone as 21854-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using NR3C2 antibody (21854-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21854-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21854-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA NR3C2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NR3C2 fusion protein Ag16410
• Vollständiger Name: nuclear receptor subfamily 3, group C, member 2
• Berechnetes Molekulargewicht: 984 aa, 107 kDa
• Beobachtetes Molekulargewicht: 94-110 kDa
• GenBank-Zugangsnummer: BC111758
• Gene symbol: NR3C2
• Gene ID (NCBI): 4306
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.