Nurr1/NR4A2 Polyklonaler Antikörper

Nurr1/NR4A2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 10975-2-AP

Synonyme

HZF 3, NOT, NR4A2, NURR1, Nurr1/NR4A2, Orphan nuclear receptor NURR1, RNR1, TINUR

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Galerie der Validierungsdaten

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  WB analysis using 10975-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10975-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10975-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 10975-2-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 10975-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 10975-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10975-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10975-2-AP (Nurr1/NR4A2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, COLO 320-Zellen, fetales humanes Hirngewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• ChIP: See 1 publications below

Produktinformation

10975-2-AP bindet in WB, IHC, IF, ChIP, ELISA Nurr1/NR4A2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Nurr1/NR4A2 fusion protein Ag1418
• Vollständiger Name: nuclear receptor subfamily 4, group A, member 2
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC009288
• Gene symbol: NR4A2
• Gene ID (NCBI): 4929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].

Publikationen

Species	Application	Title
human	WB,IHC	Cancer; Identification of low intratumoral gene expression heterogeneity in neuroblastic tumors by genome-wide expression analysis and game theory.; Authors - Albino Domenico D
mouse	IF	Cells; Hair-Follicle-Associated Pluripotent (HAP) Stem Cells Can Extensively Differentiate to Tyrosine-Hydroxylase-Expressing Dopamine-Secreting Neurons.; Authors - Michiko Yamane
human	WB	Cell Signal; miR-206 modulates lipopolysaccharide-mediated inflammatory cytokine production in human astrocytes.; Authors - Xiaodong Duan
human	IHC, ChIP	Int J Biochem Cell Biol; Caveolin-1 facilitates cell migration by upregulating nuclear receptor 4A2/retinoid X receptor α-mediated β-galactoside α2,6-sialyltransferase I expression in human hepatocarcinoma cells.; Authors - Xixi Chen
mouse	WB	Brain Sci; Itaconate Attenuates Neuroinflammation and Exerts Dopamine Neuroprotection in Parkinson's Disease through Inhibiting NLRP3 Inflammasome; Authors - Guoqing Sun
mouse	WB	Exp Ther Med; Attenuated macrophage activation mediated by microRNA-183 knockdown through targeting NR4A2.; Authors - Fu-Han Gong