Nurr1/NR4A2 Monoklonaler Antikörper

Nurr1/NR4A2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1H5B4

Kat-Nr. : 66878-1-Ig

Synonyme

HZF 3, NOT, NR4A2, NURR1, Orphan nuclear receptor NURR1, RNR1, TINUR

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Galerie der Validierungsdaten

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  WB analysis using 66878-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 66878-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66878-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 66878-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66878-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66878-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66878-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66878-1-Ig (Nurr1/NR4A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Daudi-Zellen, HT-29-Zellen, Raji-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

66878-1-Ig bindet in WB, IHC, ELISA Nurr1/NR4A2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Nurr1/NR4A2 fusion protein Ag1418
• Vollständiger Name: nuclear receptor subfamily 4, group A, member 2
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC009288
• Gene symbol: NR4A2
• Gene ID (NCBI): 4929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic mdDA neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].

Publikationen

Species	Application	Title
human	WB	Front Mol Biosci; Single-Cell Profiles of Age-Related Osteoarthritis Uncover Underlying Heterogeneity Associated With Disease Progression.; Authors - Wenzhou Liu ; KD Validated
rat	WB,IHC	Mol Cell Neurosci; BDNF-modified human umbilical cord mesenchymal stem cells-derived dopaminergic-like neurons improve rotation behavior of Parkinson's disease rats through neuroprotection and anti-neuroinflammation; Authors - Zhi Jiang
mouse	WB	World J Gastroenterol; 3,6-dichlorobenzo[b]thiophene-2-carboxylic acid alleviates ulcerative colitis by suppressing mammalian target of rapamycin complex 1 activation and regulating intestinal microbiota; Authors - Qiong-Zi He
human,rat	WB	J Pers Med; Identification and Validation of Hub Genes in the Stenosis of Arteriovenous Fistula; Authors - Yu Li