Nurr1/NR4A2 Monoklonaler Antikörper

Nurr1/NR4A2 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H5B4

Kat-Nr. : CL488-66878

Synonyme

HZF 3, NOT, NR4A2, NURR1, Nurr1/NR4A2, Orphan nuclear receptor NURR1, RNR1, TINUR

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Galerie der Validierungsdaten

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  FC experiment of HeLa using CL488-66878

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Nurr1/NR4A2 (CL488-66878, Clone:1H5B4) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66878 bindet in FC (Intra) Nurr1/NR4A2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Nurr1/NR4A2 fusion protein Ag1418
• Vollständiger Name: nuclear receptor subfamily 4, group A, member 2
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC009288
• Gene symbol: NR4A2
• Gene ID (NCBI): 4929
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR4A2 belongs to the orphan nuclear receptor (NR) family referred to as NR4A family, which have been implicated in cell cycle regulation, apoptosis, inflammation, metabolism and more recently in carcinogenesis [PMID:22583411]. NR4A2 also has a role in the expression of several proteins that are necessary for the synthesis and regulation of DA, such as tyrosine hidroxilase, DA transporter, vesicular monoamine transporter 2, and cRET [PMID:22294735]. Act as a transcriptional regulator, NR4A2 is essential for the differentiation and maintenance of meso-diencephalic mdDA neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons [PMID: 17184956].