NR5A1 Polyklonaler Antikörper

NR5A1 Polyklonal Antikörper für WB, IHC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18658-1-PBS

Synonyme

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  WB analysis using 18658-1-AP (same clone as 18658-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18658-1-AP (NR5A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18658-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 18658-1-AP (same clone as 18658-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 18658-1-AP (NR5A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18658-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A2780 using 18658-1-AP (same clone as 18658-1-PBS)

  IP result of anti-NR5A1 (IP:18658-1-AP, 4ug; Detection:18658-1-AP 1:300) with A2780 cells lysate 960ug. This data was developed using the same antibody clone with 18658-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 18658-1-AP (same clone as 18658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 18658-1-AP (same clone as 18658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 18658-1-AP (same clone as 18658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 18658-1-AP (same clone as 18658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 18658-1-AP (NR5A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 18658-1-AP (same clone as 18658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 18658-1-AP (NR5A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 18658-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary using 18658-1-AP (same clone as 18658-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 18658-1-AP (NR5A1 Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 18658-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 18658-1-AP (same clone as 18658-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NR5A1 (18658-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 18658-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18658-1-PBS bindet in WB, IHC, FC (Intra), IP, Indirect ELISA NR5A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NR5A1 fusion protein Ag13252
• Vollständiger Name: nuclear receptor subfamily 5, group A, member 1
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC032501
• Gene symbol: NR5A1
• Gene ID (NCBI): 2516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Steroidogenic factor-1 (SF-1,STF-1), also known as NR5A1, regulates multiple genes involved in the adrenal and gonadal development and in the biosynthesis of a variety of hormones, including adrenal and gonadal steroids, anti-Mullerian hormone (AMH), and gonadotropins. SF-1 belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors. Initially identified as a tissue-specific transcriptional regulator of cytochrome P450 steroid hydroxylases, research studies of both global and tissue-specific knockout mice have demonstrated that SF-1 is required for the development of adrenal glands, gonads, ventromedial hypothalamus, and for the proper functioning of pituitary gonadotropes. Indeed, humans with mutations that render SF-1 transcriptionally inactive can present with testicular failure, ovarian failure, and adrenal insufficiency. Furthermore, dysregulation of SF-1 has been linked to diseases such as endometriosis and adrenocortical carcinoma.Like other nuclear hormone receptors, SF-1 has a modular domain structure composed of an N-terminal zinc finger DNA-binding domain, a ligand-binding domain, a C-terminal AF-2 activation domain, and a hinge region with AF-1-like activation activity. SF-1 also contains a fushi tarazu factor 1 box, which functions as an accessory DNA binding domain. SF-1 is primarily phosphorylated at Ser203, which is thought to enhance its transcriptional activity by promoting complex formation with transcriptional cofactors. In addition to phosphorylation at Ser203, SF-1 is subject to SUMO conjugation and acetylation at ε-amino groups of target lysine residues. Whereas SUMOylation represses SF-1 function, acetylation enhances its transcriptional activity.In the adult ovary, SF-1 localizes to theca/interstitial cells.