NRAP Polyklonaler Antikörper

NRAP Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25977-1-PBS

Synonyme

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  WB analysis using 25977-1-AP (same clone as 25977-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25977-1-AP (NRAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25977-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25977-1-AP (same clone as 25977-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25977-1-AP (NRAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25977-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 25977-1-AP (same clone as 25977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25977-1-AP (NRAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25977-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 25977-1-AP (same clone as 25977-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 25977-1-AP (NRAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25977-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25977-1-PBS bindet in WB, IHC, Indirect ELISA NRAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NRAP fusion protein Ag23254
• Vollständiger Name: nebulin-related anchoring protein
• Beobachtetes Molekulargewicht: 190-200 kDa
• GenBank-Zugangsnummer: BC126407
• Gene symbol: NRAP
• Gene ID (NCBI): 4892
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NRAP (Nebulin-related-anchoring protein) is a striated muscle-specific protein that is concentrated at intercalated disks in cardiac muscle and at myotendinous junctions in skeletal muscle. NRAP is a 197 kDa multidomain scaffolding protein from nebulin family with many interation partners such as α-actinin, actin, vinculin and filamin (PMID:21276443). During cardiomyocyte development in the foetal heart, NRAP is involved in myofibrillar assembly and links terminal actin filaments to membrane complexes in the adult heart. Several studies observed that NRAP mutation would contributed to the dilated cardiomyopathy which is a highly heterogeneous disease with almost 100 candidates genes to date (PMID:28611399).